Xenbase Image ID: 139086

Figure 2. bCNGA1-Dend2 localizes to the PM of the rod OS. A, The localization of bCNGA1-Dend2 in X. laevis rods. B, Xenopus rod photoreceptor cell: the connecting cilium (CC) connects the IS and OS. Disks are located in the OS, and surrounded by the PM; CNGA1 localization is denoted by the dashed red lines. C–E, Phalloidin-stained bCNGA1-Dend2 transgenic Xenopus retina indicates the CPs are enriched with F-actin filaments (red). CPs do not contain bCNGA1-Dend2 (green). E, Retinas from bCNGA1-Dend2 transgenic tadpoles were pretreated with neutral protease dispase and BAPTA to partially destroy CP–OS connections (arrowheads). F, The localization of bCNGA1-Dend2 (top and middle) in PM and peripherin/rds-Dend2 (P/rds-Dend2, bottom) in disk rims and incisures of X. laevis rods. The middle represents the region transecting axoneme, around which bCNGA1-Dend2 is absent. G, Localization of bCNGA1-Dend2 in PM (top), rhodopsin-Dend2–1D4 (Rho-Dend2–1D4) in PM (arrow) and disks (asterisk; middle), and peripherin/rds-Dend2 (P/rds-Dend2) in disk rims (bottom) in hypotonically lysed retinas. Images are maximum projections of optical slices (A, right, C, E) or single confocal images (A, left, D, F, G). Images are either from live and unfixed retina (A); fixed retina (C–F); or hypotonically lysed, unfixed retina (G). Images (D) and (F) were processed by 2D blind deconvolution, to deblur the images and to reveal bCNGA1-Dend2-enriched structures (arrowheads). Animals were 14 d old. Scale bars: A, C, E, G, 5 μm; D, F, 2 μm. Image published in: Nemet I et al. (2014) Copyright © 2014. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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