Xenbase Image ID: 139089

Figure 5. bCNGA1-Dend2 mobility in the PM of the rod OS. A, The photoconversion technique allows discrimination of old (red) from newly synthesized (green) bCNGA1-Dend2. Forty-eight hours after photoconversion, newly added bCNGA1-Dend2 was concentrated at the basal portion of the rod OS where newly formed disk membranes are added (Lodowski et al., 2013). Images are from live and unfixed retina. Scale bar, 5 μm. B, Lateral and longitudinal diffusion of rhodopsin-Dend2–1D4 and longitudinal diffusion of bCNGA1-Dend2 were studied by FRAP in X. laevis rods. FRAP measurements were corrected and fitted to curves to estimate the diffusion coefficients (D) and percentage of immobile fraction as described in Materials and Methods. A.U., arbitrary units. Image published in: Nemet I et al. (2014) Copyright © 2014. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

Return to previous page