Xenbase Image ID: 139092

Figure 8. Evagination overgrowths induced by F-actin disruption contain rhodopsin and peripherin/rds. The photoconversion technique allows discrimination of old (red) from newly synthesized (green) proteins. Xenopus rods were imaged after cytochalasin D, latrunculin A, or DMSO control treatment for 24 h. A, In cytochalasin D- and latrunculin A-treated rods, both old (red) and new (green) rhodopsin-Dend2–1D4 are observed in the evagination overgrowths (arrows). In control rods, evagination overgrowths are not observed, and new rhodopsin-Dend2–1D4 (green) is incorporated into new disks. B, In cytochalasin D- and latrunculin A-treated rods, new peripherin/rds-Dend2 (green) is observed in the evagination overgrowths (arrows). Old peripherin/rds-Dend2 (red) is not observed in these structures (arrows). In control rods, evagination overgrowths are not observed, and new peripherin/rds-Dend2 (green) is incorporated into new disks. Intensity profile charts (A, B, right) were drawn for old and newly added proteins along the lines on left images. Rectangles in the charts represent the areas of evagination overgrowths (arrows). Animals were 14 d old at the time of photoconversion. Images are from live and unfixed retinas. Scale bars: 5 μm. A.U., arbitrary units. Image published in: Nemet I et al. (2014) Copyright © 2014. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

Return to previous page