Xenbase Image ID: 139093

Figure 9. Differential targeting of rhodopsin, peripherin/rds, and CNG channel to the OS compartments. Schematic showing the summary of photoconversion experiments in this study. a, Disk (rhodopsin), disk rim (peripherin/rds), and PM (CNG channel) protein trafficking in rod photoreceptor cell. According to the evagination hypothesis, rhodopsin is trafficked to evaginations, whereas peripherin/rds is not. CNG channels are incorporated into the OS PM as disk membranes are formed. Proteins are trafficked from the IS to the OS. b, Photoconversion turns Dend2 fusion proteins from green to red. This conversion is irreversible, and thus old proteins stay red. c, After F-actin disruption, rhodopsin-Dend2–1D4 and peripherin/rds-Dend2 are trafficked to the evagination overgrowths, while trafficking of the bCNGA1-Dend2 to the OS PM is inhibited. The presence of old and photoconverted rhodopsin-Dend2–1D4 in the evagination indicates that the evagination was the original destination of rhodopsin-Dend2–1D4 (compare b and c). The lack of old and photoconverted peripherin/rds-Dend2 in the evagination indicates that the evagination was not the original destination (compare b and c). Image published in: Nemet I et al. (2014) Copyright © 2014. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

Return to previous page