Xenbase Image ID: 139099

Fig. 1. Custos is required for anterior development. (A) Schematic representation of Custos and its domains. Arrowheads indicate the two clusters of dual CKI phosphorylation sites. (B) Custos-MO blocks translation of tagged-Custos protein and depletes endogenous Xenopus Custos protein levels. Myc-tagged Custos RNA containing the MO binding site was coinjected with control MO or Custos-MO, and tagged-protein was visualized by SDS/PAGE and Western blotting (Left). Endogenous Custos protein was assessed using anti-Custos antibody (Right). (C) Depletion and overexpression of Custos induce anterior head defects. The indicated RNA or MO was injected into the two dorsal blastomeres of 4-cell embryos. (D) Suppression of head development was scored by the dorso-anterior index (DAI). The numbers above each bar indicate the number of injected embryos. Image published in: Komiya Y et al. (2014) Copyright © 2014. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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