Xenbase Image ID: 139101

Fig. 3. Custos inhibits Wnt signal transduction. (A) Topflash reporters were cotransfected into HEK-293T cells with expression plasmids for Wnt1, Dvl or β-catenin and Custos, as indicated. (B) Topflash reporter was coinjected with Wnt8, Dsh, or β-catenin and Custos RNAs into all cells of two-cell embryos. Cell or embryo lysates (stage 10.5) were subjected to luciferase assay and normalized to reporter-only samples. Image published in: Komiya Y et al. (2014) Copyright © 2014. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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