Xenbase Image ID: 139107

Fig. S4. Temporal and spatial expression pattern of Custos in Xenopus embryo. (A) Xenopus Custos is expressed throughout development. The expression level of Custos RNA was assessed by RT-PCR analysis. Ornithine Decarboxylase (ODC) was used as an internal control, RT(−) is without reverse transcriptase as a negative control. (B) Custos is highly expressed in the anterior region of the embryo. Whole-mount in situ hybridization was performed using a Xenopus Custos anti-sense RNA probe. No signal was detected using a Custos sense RNA probe. (C) Expression of Custos does not induce or suppress levels of activated RhoA in HEK-293T cells; the GEF Ephexin is used as a positive control. (D) Sequence of Xenopus Custos antisense morpholino oligonucleotide (MO) is indicated by red letters. Image published in: Komiya Y et al. (2014) Copyright © 2014. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue c1h12orf43.L c12orf43, custos X. laevis Throughout unfertilized egg stage egg c1h12orf43.L c12orf43, custos X. laevis Throughout NF stage 9 animal hemisphere c1h12orf43.L c12orf43, custos X. laevis Throughout NF stage 12 ectoderm neuroectoderm non-neural ectoderm c1h12orf43.L c12orf43, custos X. laevis Throughout NF stage 15 neuroectoderm neural plate pre-chordal neural plate chordal neural plate neural plate border neural crest premigratory neural crest cell c1h12orf43.L c12orf43, custos X. laevis Throughout NF stage 20 neural plate pre-chordal neural plate chordal neural plate neural plate border optic vesicle neural crest cranial neural crest anterior placodal area c1h12orf43.L c12orf43, custos X. laevis Throughout NF stage 23 neural tube posterior neural tube anterior neural tube dorsolateral neural tube ventrolateral neural tube neural crest cranial neural crest optic vesicle anterior placodal area c1h12orf43.L c12orf43, custos X. laevis Throughout NF stage 29 and 30 eye optic vesicle otic vesicle neural crest cranial neural crest branchial arch mandibular crest hyoid crest mandibular arch hyoid arch spinal cord brain forebrain midbrain hindbrain c1h12orf43.L c12orf43, custos X. laevis Throughout NF stage 35 and 36 spinal cord otic vesicle brain forebrain midbrain hindbrain eye head mesenchyme neural crest cranial neural crest Image source: Published Larger Image Printer Friendly View

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