Xenbase Image ID: 139297

Fig. 2. Temporal and spatial expression pattern of BENI during Xenopus development. (A) Temporal expression pattern of BENI during Xenopus development detected by RT-PCR. RT-PCR using an ornithine decarboxylase (ODC)-specific primer was carried out in parallel to control the amount of input RNA [ODC]. RT-PCR without reverse transcriptase showed no contamination of genomic DNA [ODC(RT−)]. (B–Q) Spatial expression pattern of BENI during development detected by whole-mount in situ hybridization. Embryos were hybridized with antisense (C, D, F, G, I, K, M–P, Q) and sense probes (B, E, H, J, L). (B, C) Stage 11, animal view. (D) Stage 11, vegetal view. (E, F) Stage 17, lateral view. (G) Stage 17, anterior view. (H, I) Stage 22, lateral view. (J, K) Stage 29/30, lateral view. (L, M) Stage 40, lateral view. (N–S) Transverse section of the embryo at stage 10.5. Panels P and Q are magnified views of panels N and O, respectively. Xbra expression (R–S). Scale bars, 100 μm. Image published in: Homma M et al. (2007) Copyright © 2007. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue znf750.L BENI, sg1, znf750-a, znf750-b X. laevis Throughout NF stage 11 animal hemisphere ectoderm znf750.L BENI, sg1, znf750-a, znf750-b X. laevis Throughout NF stage 17 ectoderm epidermis non-neural ectoderm cement gland primordium znf750.L BENI, sg1, znf750-a, znf750-b X. laevis Throughout NF stage 22 ectoderm epidermis non-neural ectoderm hatching gland cement gland primordium znf750.L BENI, sg1, znf750-a, znf750-b X. laevis Throughout NF stage 29 and 30 epidermis hatching gland cement gland znf750.L BENI, sg1, znf750-a, znf750-b X. laevis Throughout NF stage 40 epidermis head fin znf750.L BENI, sg1, znf750-a, znf750-b X. laevis Throughout NF stage 10.5 animal cap tbxt.S bra, brachyury, ntl, t, t-a, t-b, X-bra, Xbra, Xbrachyury X. laevis Throughout NF stage 10.5 involuting marginal zone Image source: Published Larger Image Printer Friendly View

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