Xenbase Image ID: 139298

Fig. 3. Activity of BENI antisense morpholino oligonucleotide (BENI-MO). (A) BENI-EGFP (300 ng) or BENImut-EGFP (300 ng) was coinjected with BENI-MO (4 pmol = 34 ng) in the animal pole of Xenopus embryos. The embryos were raised until stage 9 and the EGFP was visualized and observed under a fluorescent microscope (A) and animal caps of the embryos were harvested for SDS-PAGE analysis. The animal cap lysates were subjected to SDS-PAGE and immunoblotted with anti-GFP and anti-α-tubulin (B). (C) Injection of BENI-MO (4 pmol = 34 ng) at both dorsal animal blastomeres of 4-cell embryos. The embryos were observed at stage 37/38. Scale bars, 1 mm. Image published in: Homma M et al. (2007) Copyright © 2007. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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