Xenbase Image ID: 139299

Fig. 4. Gastrulation defect caused by BENI mRNA. (A) lacZ mRNA (400 pg), BENI mRNA (1 ng) and lacZ mRNA, or BENI-MO (4 pmol = 34 ng) and lacZ mRNA were injected into both dorsoanimal blastomeres of 4-cell embryos. Vegetal view of embryos at stage 10 (top panels). There was no discernible delaying of gastrulation. Embryos were histologically examined and observed under diffuse light (middle panels). High magnification view of dorsal involuting marginal zone (IMZ) of the middle panels (bottom panels). Double-headed arrow indicates width of dorsal IMZ. Yellow arrowheads indicate the dorsal lip. Scale bar, 200 μm. (B) Width of dorsal IMZ was measured. (C) lacZ mRNA or BENI mRNA and lacZ mRNA was injected into both ventroanimal blastomeres of 4-cell embryos. Embryos were bisected at stage 10 (top panels). Highly magnified ventral IMZ of top panels (bottom panels). Double-headed arrow indicates width of ventral IMZ. Scale bar, 200 μm. (D) Width of ventral IMZ was measured. (E) lacZ mRNA (control, upper column), lacZ mRNA and BENI MO (middle column), and lacZ mRNA and TSC-22 mRNA (lower column) were injected into dorsoanimal blastomeres of 4-cell embryos. At stage 13, embryos were stained with Red-gal and the staining pattern observed. Image published in: Homma M et al. (2007) Copyright © 2007. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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