Xenbase Image ID: 139309

FIG. 1. Melanocytes and neural markers are induced in noggin-dorsalized VMZ explants. In a representative experiment, 36 VMZ explants were removed from stage 10/ to 10.25 embryos. Eighteen explants were noggin-treated until stage 12.5–13 and the 18 remaining explants served as controls. All explants were grown to stage 41 and scored for melanocytes. Approximately 75% of the noggin-treated VMZs made melanocytes in comparison to 5% of the controls. Embryos were fixed in MEMFA according to Hemmati-Brivanlou et al. (1990a) and cleared in 2:1 benzyl benzoate:benzyl alcohol before photography. (A) Six representative noggin-dorsalized VMZ explants. (B) Six representative control VMZ explants. (C) Induction of neural markers in dorsalized VMZ explants. Eighteen VMZ explants were removed from embryos at stage 10/ to 10.25. Nine explants were noggin-treated until stage 12.5–13, and 9 untreated explants served as controls. Explants were grown to several stages (20, 26, 36, and 41), and total RNA was isolated for Northern analysis. Total RNA was also isolated from pools of five whole embryos. All 9 explants and one embryo equivalent of RNA were loaded per well. Filters were sequentially hybridized with Xenopus cDNA probes for muscle-specific actin (m-actin), XMyoD, NCAM, nrp-1, XIF-3, n-tubulin, Hoxb9, XAG-1, E13 (epidermal cytokeratin), and EF1a. The results from stage 26 explants are shown; similar results (not shown) were observed at stages 20, 36, and 41. All data were quantitated as described under Materials and Methods. Image published in: Bonstein L et al. (1998) Copyright © 1998. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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