XB-IMG-139311
Xenbase Image ID: 139311
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FIG. 3. Paraxial-fated mesoderm is a strong inducer of neural crest cells. Stage 10.25–10.5 DLMZs and DMZs were removed from
homozygous albino embryos and recombined with blastula stage ACs. DLMZs, DMZs, and ACs were also removed in parallel as controls.
In a routine experiment, 18 explants from each group were scored for melanocytes at stage 41, and 9 explants from each group were grown
until stage 17 for Northern analysis. (A) Three representative DLMZ–AC recombinant explants are shown. (B) Three representative DMZ–
AC recombinant explants are shown. (C) Northern analysis of recombinant DLMZ and DMZ explants. Nine explant equivalents and one
embryo equivalent (from a pool of five control embryos) of RNA were loaded per well. Filters were sequentially hybridized with Xenopus cDNA probes for XSlug, XAG-1, nrp-1, muscle-specific actin (m-actin), and EF1a. On all filters, the muscle-specific actin is the lower
band; the upper band is cross-hybridization to cytoskeletal actin. (D) Whole-mount in situ hybridization with XSlug was performed on
mid-neurula-stage embryos and explants (see Materials and Methods). Top: XSlug expression in a mid-neurula-stage embryo injected with
b-gal, dorsal view. Bottom: DLMZ–AC recombinant explants in which AC cells were injected with b-gal; the arrows show the purple
alkaline phosphatase staining on the red b-gal background. Image published in: Bonstein L et al. (1998) Copyright © 1998. Image reproduced with permission of the Publisher, Elsevier B. V.
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