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Figure 1. MIXL1 expression confers decreased sensitivity to doxorubicin in AML cells(A) Stable transfectants of U937 cells express MIXL1 at levels similar to those of endogenous MIXL1 in AML cell lines. MIXL1 was detected by probing 30 μg of whole cell lysates resolved on SDS-PAGE and transferred to PVDF membrane, with rabbit antibodies against N-terminal epitope of MIXL1 and with β-actin for a loading control [17]. (B) MIXL1 expression reduces sensitivity of U937 cells to doxorubicin. The cell lines were treated with 0–1.75 μM doxorubicin on day 0. Cell survival was measured at 24 hours by MTS assay as detailed in Materials and Methods. Absorbance of untreated cells was normalized to 1. Relative viability at varying concentrations of doxorubicin is denoted. Image published in: Raymond A et al. (2014) Copyright: © 2014 Raymond et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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