Xenbase Image ID: 139530

Figure 4. MIXL1 binds to the c-REL promoter(A) c-REL promoter peak region identified by ChIP-Seq, as generated by the University of California, Santa Cruz, genome browser is shown. The location and size of each promoter fragment used for the luciferase reporter assay is displayed underneath. (B) MIXL1 binds to a 550-bp region within the c-REL promoter. Regions of the DNA depicted in 4A were cloned into the reporter vector pBV-Luc luciferase, which were then transiently co-transfected into HEK293T cells with MIXL1, MIXL1 Homeobox-less, or empty expression vector. Equal amount of Renilla luciferase co-transfected with the reporter constructs allowed normalization. Luciferase activity of each combination was tested in triplicate after 48 hours. Error bars represent standard deviation between triplicates. (C) MZF1 binds to the same locus as MIXL1 on c-REL promoter. Quantitative PCR analysis of the identified c-REL promoter region and c-REL intron control region compared the abundance of each genomic locus immunoprecipitated by either IgG or MZF1 antibodies, normalized to a standard curve. Error bars represent standard deviation between triplicates. *p < 0.05. (D) Model depicting a potential MIXL1-TBX-MZF1 multiprotein complex activating c-REL transcription. Image published in: Raymond A et al. (2014) Copyright: © 2014 Raymond et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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