Xenbase Image ID: 139630

Figure 1. E2a Is Required for Subsets of Smad2/3 Binding and for Global Patterns of Smad2/3 Target Gene Expression (A) Smad2/3 targets 1,027 distinct genomic regions in control stage 10.5 embryos (yellow) and 1,671 regions in E2a-depleted embryos (blue). Four hundred ninety-five regions are targeted in both conditions. (B) Categories of Smad2/3 binding behavior in E2a-depleted embryos (blue) relative to controls (yellow). (C) DAVID clustering analysis shows enrichment for developmental (dev) terms in all Smad2/3-associated genes and for genes that have stable Smad2/3 binding when E2a is depleted (red box in B) but not other subcategories of Smad2/3 binding. Ant/Post, anterior/posterior. (D) Genes that retain Smad2/3 binding at the same genomic coordinates in E2a-depleted embryos (red box in B) are more likely to be downregulated by 2-fold or more in E2a-depleted embryos. (E and F) Significant overlap exists between genes that are Smad2/3 targets and genes that are downregulated in E2a-depleted embryos. Smad2/3 targets in which binding is at the same genomic position in control and E2a-depleted embryos are more likely to be downregulated in E2a-depleted embryos. These genes maintain enrichment for DAVID terms associated with early development. Image published in: Wills AE and Baker JC (2015) Copyright © 2015. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

Return to previous page