XB-IMG-139631
Xenbase Image ID: 139631
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Figure 2. E2a Regulates Samd2/3 Positioning at lefty and Represses lefty Transcription (A) qRT-PCR showing upregulation of lefty in E2a-depleted embryos and downregulation of gsc and xbra. odc is shown as a loading control (Ctrl). Error bars represent SDs for three biological replicates.
(B) In situ hybridization for lefty expression at stage 10 in control, E2a-depleted, and e2a mRNA-injected embryos (red, lacZ lineage tracer).
(C) Distribution of key transcription factor binding sites near the lefty locus. There are two regions of observed Smad2/3 binding in control embryos, schematized in blue.
(D) In E2a-depleted embryos, Smad2/3 binding at region 1 is reduced, while binding at region 2 is increased. Fold enrichment over input is shown along the y axis.
(E) Embryos were injected at the two-cell stage with mE2a-GFP- and RFP-tagged histone H2b mRNA and monitored for fluorescence at stage 10.5.
(F) ChIP-qRT-PCR using anti-GFP antibodies in stage 10.5 embryos following mE2a-GFP injection at the two-cell stage. The y axis represents fold enrichment over a negative (neg) control region near the lefty locus. Error bars represent SDs of two to three biological replicates.
See also Figure S1. Image published in: Wills AE and Baker JC (2015) Copyright © 2015. Image reproduced with permission of the Publisher, Elsevier B. V.
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