Xenbase Image ID: 139632

Figure 3. Lefty Is Downstream of E2a in Mesoderm Induction (A) Embryos were injected with E2a MO in specific blatomeres as follows: All, both blastomeres at the two-cell stage (n = 72); Dorsal, two dorsal blastomeres at the four-cell stage (n = 51); Ventral, both ventral blastomeres at the four-cell stage (n = 63); Animal, four animal blastomeres at the eight-cell stage (n = 9). (B) Embryos were injected in both blastomeres at the two-cell stage with e2a MO and/or at the animal pole at the four-cell stage with xnr1 mRNA. Animal caps were harvested at stage 8 and cultured to stage 10. qRT-PCRs were normalized to whole embryo expression and to odc expression. Error bars represent SDs for three biological replicates. (C–J) E2a-depleted embryos have reduced expression of gsc (E) and xbra (F) compared with uninjected control embryos (C and D). By contrast, Lefty-depleted embryos show increased expression of gsc (G) and xbra (H). Embryos injected with both E2a and Lefty MOs have moderate expression of gsc (I) and xbra (J). See also Figure S2. Image published in: Wills AE and Baker JC (2015) Copyright © 2015. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue gsc.L goosecoid, gsc-a, gsc-b, Xgsc X. laevis Throughout NF stage 10 mesoderm tbxt.S bra, brachyury, ntl, t, t-a, t-b, X-bra, Xbra, Xbrachyury X. laevis Throughout NF stage 10 mesoderm Image source: Published Larger Image Printer Friendly View

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