Xenbase Image ID: 139637

Supplemental Figure S2, related to Figure 3. Expression of lefty in E2a depleted embryos is not high enough to cause loss of bottle cells. A) Dose curve of phenotypic effects of lefty mRNA injection. Increasing levels of lefty mRNA injection (both dorsal blastomeres at the 4-cell stage; total mRNA dose shown) result in progressively more severe inhibition of bottle cell and blastopore formation. At 1pg total lefty mRNA, blastopore formation is not inhibited, and at 2.5pg, it is only modestly inhibited, while blastopore formation is significantly impaired at 10pg or more of lefty mRNA. B) qRT-PCR comparing the effects of E2a MO injection with lefty mRNA injection at a range of doses. The amount of injected lefty mRNA is indicated across the top of the graph. The amount of lefty mRNA expressed in E2a morphants is comparable to injection of between 1 and 2.5pg lefty mRNA. Image published in: Wills AE and Baker JC (2015) Copyright © 2015. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

Return to previous page