Xenbase Image ID: 139645

Fig. 6. In pre-ZLI-like/thalamus-like explant sandwiches, cells recapitulate the main features of their in vivo developmental program. (A, B) In sandwiched explants pre-ZLI-like cells segregate and form a boundary with thalamus-like cells in the presence of N-Shh. (a) Experimental design: cRNA were injected as indicated. (b, c) Representative explants of thalamus-like AACs (red) sandwiched with pre-ZLI-like AACs without (A) or with (B) N-Shh. (A) In the absence of N-Shh pre-ZLI-like cells did not express Xshh and intermingled with thalamic-like cells. (c) Enlarged views and (d) section, of a representative explant. In contrast (B) in the presence of N-Shh pre-ZLI-like cells expressed Xshh and formed a boundary with thalamus-like cells (85%, n=15). (c) Enlarged views and (d) section, of a representative explant. Image published in: Juraver-Geslin HA et al. (2014) Copyright © 2014. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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