Xenbase Image ID: 139647

Fig. 8. Some patterning defects observed in Barhl2 depleted embryos are rescued by decreasing ß-catenin levels, but not ZLI development. (A–D) ISH on embryos depleted for barhl2 (a, b) or both barhl2 and ßcatenin (c) using pax6 (A), irx1 (B), irx2 (C), and shh (D) as probes. The CT (MObarhl2) and rescued (MObarhl2/MOßcat) embryos were generated during the same set of experiments, and were coinjected with a MO control used as tracer, coupled to fluorescein detected by immunochemistry (red). For MObarhl2/MOßcat injected embryos, only the injected side is shown. The scale bar stands for 0.2 mm. (E–I) Barhl2 depletion patterning defects are partly rescued by decreasing ß-catenin levels. Barhl2 depletion phenotypic defects, specifically (A) pax6 ectopic expression in the mid-diencephalic furrow (n=25), (B, C) the decrease of irx1 (n=20) and irx2 (n=22) thalamic expression are partly rescued by depletion of ßcat. (D) The loss of ZLI development observed in Barhl2-depleted embryos is not rescued by decreasing ß-catenin levels. At st. 37 the progression of shh inside the p2 alar plate is inhibited in both Barhl2 depleted embryos (b, n=36) and Barhl2/ßCat double morphants embryos (c, n=36). Image published in: Juraver-Geslin HA et al. (2014) Copyright © 2014. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue shh.L hedgehog, vhh-1, X-SHH, Xhh, xshh X. laevis Throughout NF stage 35 and 36 zona limitans intrathalamica basal forebrain irx2.L Iro2, MGC89388, Xiro2 X. laevis Throughout NF stage 32 thalamus diencephalon pax6.L an2, mgda, pax-6, pax6-a, pax6-b, wagr, XLPAX6, xpax6 X. laevis Throughout NF stage 27 forebrain pretectum irx1.L iro, irx1-a, irx1-b, Xiro, Xiro1 X. laevis Throughout NF stage 31 forebrain thalamus Image source: Published Larger Image Printer Friendly View

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