Xenbase Image ID: 139655

Figure S2. Related to Figure 2. Sox5 misexpression and knockdown causes gene expression defects in gastrula stage embryos. (A) phospho-Histone H3 staining of stage 15 embryos injected with Sox5 mRNA or Sox5 morpholino. No differences in proliferation are seen. (B) TUNEL staining stage 15 embryos injected with Sox5 mRNA or Sox5 morpholino. No differences in apoptosis are seen. DNMT3bmediated apoptosis serves as a positive control. Asterisk denotes side with β-gal staining (red) lineage tracer. (C) In situ hybridization examining expression of MyoD in stage 15 embryos injected with Sox5 mRNA or Sox5 morpholino in micromeres at the 8-cell stage targeting for ectodermal tissue that will give rise to neural crest. No defects in mesoderm are noted. (D) In situ hybridization examining expression of MyoD in stage 15 embryos injected with Sox5 mRNA or Sox5 morpholino in macromeres at the 8 cell stage targeting for mesoderm. Mesoderm formation as assayed by MyoD expression is perturbed, reflecting a role for Sox5 in this tissue. (E) In situ hybridization examining expression of Sox8 in embryos injected with Sox5 mRNA. Embryos were collected at both stage 17 and stage 23. (F) In situ hybridization examining expression of FoxD3 and Snail2 in embryos injected with Sox5* (a shorter Sox5 isoform/splice variant expressed in laevis). Embryos were collected at mid-neurula stages, stage 17. (G) In situ hybridization examining expression of EPK and Sox3 in embryos injected with Sox5*. Embryos were collected at early-neurula stages, stage 14. (H) In situ hybridization examining expression of Msx1, Pax3 and Zic1 in embryos injected with Sox5*. Embryos were collected at earlyneurula stages, stage 14. Sox5* behaved indistinguishably to Sox5 in these assays. (I-K) In situ hybridization examining expression of Id3 (I), AP2 (J) and Pax3 (K) at gastrula stages (stage 11.5) in embryos injected with Sox5 or Sox5MO. Red arrow denotes blastopore. Asterisk denotes injected side. Image published in: Nordin K and LaBonne C (2014) Copyright © 2014. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue h3-3a.L h3.3, h3.3a, h3f3a X. laevis Throughout NF stage 15 mitotic cell myod1.S mf25, MyoD, myod1-a, myod1-b, myogenic factor 3, XMyoD, XtmyoD X. laevis Throughout NF stage 15 paraxial mesoderm sox8.L X. laevis Throughout NF stage 17 cranial neural crest foxd3.L fkh6, foxd3-a, foxd3-b, XFD-6, xfoxd3 X. laevis Throughout NF stage 17 cranial neural crest snai2.L slug, snai2-a, snai2-b, Snail2, xSlu, xslug, XSnail2 X. laevis Throughout NF stage 17 cranial neural crest tfap2a.L ap2, AP2a, AP2alpha, tfap2a-a, tfap2a-b, tfap2alpha, XAP-2, xap2 X. laevis Throughout NF stage 11.5 animal hemisphere id3.L heir-1, id3-a, id3-b, xid3, xidia, xIdx X. laevis Throughout NF stage 11.5 animal hemisphere pax3.S cdhs, hup2, pax-3, pax3-a, pax3-b, Waardenburg syndrome 1, ws1, xpax3 X. laevis Throughout NF stage 11.5 neural plate border krt12.4.L ck81, cytok, cytokeratin, E-keratin, epi-keratin, epidermal keratin, Epidermis-specific keratin, EpiK, epiker, EpK, epK, K81, xK81, xk81a, xk81a1 X. laevis Throughout NF stage 17 non-neural ectoderm zic1.S odd-paired-like, opl, Zic-r1, Zic-related-1 X. laevis Throughout NF stage 17 anterior neural ridge sox3.S Sry, Xlsox3, xSox3, Xtsox3 X. laevis Throughout NF stage 17 neural plate Image source: Published Larger Image Printer Friendly View

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