Xenbase Image ID: 139668

Fig. 2. Suppression of Lrig3 by a splicing morpholino disturbed embryonic development. (A) RT-PCR shows that injection of L3MO decreased the mature Lrig3 mRNA while inducing the predicted ∼1 kb unspliced band, using intron-spanning primers for pseudoalleles Lrig3A and Lrig3B. ODC was loading control. (B-D) Phenotypes induced by L3MO. The morphants showed severe anterior defects, delayed or failed neural fold closure, and shortened axis. (C,D) Embryos were injected with 30 ng L3MO; uninjected control in B. (E-G) The phenotype was rescued by co-injection of Lrig3 mRNA. (E) Uninjected control; (F) L3MO (30 ng); (G) 30 ng of L3MO plus 20 pg Lrig3 mRNA. Image published in: Zhao H et al. (2008) Copyright © 2008. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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