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Fig. S5. Injection of L3MO inhibited trigerminal nerve formation. One dorsal blastomere of 4-cell stage embryos was injected with either control MO (A,C) or L3MO (B,D), and allowed to develop to stage 35. Two branches of the trigeminal nerve, the ophthalmic and mandibular, were visualized by whole-mount in situ hybridization with Synuclein γ. The expression of Synuclein γ was reduced on the Lrig3-depleted side (77%, 20 of 26 embryos), while embryos injected with control MO showed normal expression (92%, 12 of 13). Image published in: Zhao H et al. (2008) Copyright © 2008. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue sncg.S bcsg1, gamma-synuclein, sncg-a, sncg-b X. laevis Throughout NF stage 35 and 36 pineal gland trigeminal ganglion spinal cord roof plate cement gland Image source: Published Larger Image Printer Friendly View

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