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Figure 4. The K346T mutation increases protein stability. (A) WB analysis of protein extracts derived from cells expressing WT and K346T channels treated with the protein synthesis inhibitor cycloheximide for 3, 6 and 12 h. WT protein degradation is almost complete after 12 h treatment, while K346T protein is still detectable at this time. Actin is used as loading control. Molecular weight markers are on the left (kDa). (B) Densitometric analysis of protein bands normalized with respect to the amount of either WT (white bar) or K346T (gray bar) Kir2.1 protein in control conditions. Data are expressed as mean ± SEM from four independent experiments (***P < 0.001). Image published in: Ambrosini E et al. (2014) © The Author 2014. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |