Xenbase Image ID: 139756

Figure 5. The K346T mutation affects the distribution of Kir2.1 channels in membrane lipid rafts. (A) WB analysis of cholesterol-rich (triton insoluble fractions: 3–5) and cholesterol-poor membrane fractions (triton soluble fractions: 10–12) of WT or K346T Kir2.1-expressing cells. WT channels are mainly distributed in triton insoluble fractions (gray box), whereas K346T is also abundantly localized in cholesterol-poor fractions (black boxes). Cav-1 and flotillin-1 identify the caveolar raft fractions. Molecular weight markers are on the left (kDa). (B–E) Normal distributions of total protein (indicated on top) in membrane fractions isolated by sucrose density gradient. The levels of protein in each fraction are normalized to the total protein amount recovered from all the fractions together. Image published in: Ambrosini E et al. (2014) © The Author 2014. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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