Xenbase Image ID: 139757

Figure 6. The K346T mutation reduces Kir2.1 channels interaction with Cav-2. (A) WB analysis of Kir2.1 channel's interactors after Histidine (His) co-purification of astrocytoma cells expressing WT or K346T channels. Input lanes represent protein extracts before His co-purification. WT and K346T channels co-purify similarly with Cav-1, whereas the K346T mutation reduces the association with Cav-2. One representative experiment out of three is shown. Molecular weight markers are indicated on the left (kDa). (B) Densitometric analysis (ratio) of protein bands corresponding to Cav-1 and Cav-2 normalized respect to WT (white bars) or K346T (gray bars) Kir2.1 protein levels. Data are expressed as mean ± SEM from three independent experiments (***P < 0.001). Image published in: Ambrosini E et al. (2014) © The Author 2014. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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