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Figure 3. Grafts of ShhC25II-soaked beads reproduce the stage-dependent effects of PMP treatments on eye DV patterning. (A) Lateral views of heads of st. 33 embryos that received a graft of control or ShhC25II-soaked beads next to the optic vesicle at the indicated stages and hybridized with probes for Pax2, Vax1b or Vax2. Compared to controls, embryos grafted with ShhC25II beads show stage-dependent increase in the expression domains of ventral eye genes. Scale bar, 100 μm. (B) Quantification of the percentages of embryos with different effects on gene expression domains or eye reductions (S) in each treatment condition. The number of experiments performed for each probe and treatment condition is indicated on top of the corresponding histogram bar. (C) Histological sections of eyes of st. 33 embryos treated as in (A) and (B) and hybridized with the indicated probes, confirming stage dependent effects on DV eye patterning as detected in whole mount views. Triangles point to ectopic Pax2 expression in the dorsal marginal zone and arrows to expanded ventral expression domains of Pax2, Vax1b and Vax2, in embryos grafted ShhC25II beads. Stars indicate the position of the beads. Scale bar, 100 μm. Image published in: Wang X et al. (2015) © Wang et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue pax2.L LOC108697493, pax-2, pax2-a, pax2-b, XPax-2, XPax2 X. laevis Throughout NF stage 33 and 34 eye optic stalk retina ventral vax1.L vax, vax1-a, vax1-b, vax1b, vax1b-a, xvax1, xvax1b X. laevis Throughout NF stage 33 and 34 eye optic stalk retina ventral vax2.L dres93, vax2-a, vax2-b, vax3, xvax2 X. laevis Throughout NF stage 33 and 34 eye optic stalk retina ventral Image source: Published Larger Image Printer Friendly View

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