Xenbase Image ID: 139927

Figure 1. Nuclear export of SRP RNA is independent of CRM1. (A) A mixture of in vitro-transcribed 32P-labeled DHFR mRNA, Xenopus SRP RNA, U1ΔSm RNA, U6Δss RNA and tRNAphe was injected into the nucleus of Xenopus oocytes either with 0.2 μg/oocyte of BSA-NES (lanes 3 and 4) or with the same amount of BSA-mut (mutated NES, lanes 5 and 6). DHFR mRNA and U1ΔSm RNA were m7G-capped, and other RNAs were uncapped. RNA was analyzed immediately (0 h; lanes 1 and 2) or 1.5 h (1.5 h; lanes 3–6) after injection. N, nuclear; C, cytoplasmic fractions. (B) Quantitation of RNA export from four independent experiments as in A. (C) The same as A except that human SRP RNA instead of Xenopus SRP RNA was injected into the nucleus of oocytes either with 0.3 μg/oocyte of BSA-NES or with the same amount of BSA-mut. (D) Quantitation of RNA export from three independent experiments as in C. Image published in: Takeiwa T et al. (2015) © 2015 The Authors. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives license Larger Image Printer Friendly View

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