XB-IMG-139928
Xenbase Image ID: 139928
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Figure 2. SRP RNA shares the same export pathway with pre-microRNA and tRNA. (A) A mixture of 32P-labeled Xenopus SRP RNA, U1ΔSm RNA, U6Δss RNA and human pre-miR-31 was injected into the nucleus of oocytes either alone (lanes 2 and 3) or with increasing amounts (0.2, 0.5, and 1.0 pmol/oocyte) of unlabeled pre-miR-31 (lanes 4–9). RNA was analyzed immediately (total; lane 1) or 40 min (40 min; lanes 2–9) after injection. (B) The same as A except that 32P-labeled tRNAphe and unlabeled tRNAphe (0.2, 1.0, and 5.0 pmol/oocyte) were used instead of pre-miR-31, and the incubation time was 0 or 30 min. (C) The same as A except that unlabeled U1ΔSm RNA (0.5, 1.0, and 2.0 pmol/oocyte) was used instead of unlabeled pre-miR-31. Image published in: Takeiwa T et al. (2015) © 2015 The Authors. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives license Larger Image Printer Friendly View |