Xenbase Image ID: 139930

Figure 4. Endogenous exportin-5 mediates nuclear export of SRP RNA. (A) A mixture of 32P-labeled Xenopus SRP RNA, U1ΔSm RNA, U6Δss RNA, tRNAphe and pre-miR-31 was injected into the nucleus of oocytes either alone (lanes 2 and 3) or with 38 ng/oocyte of anti-Exportin-5 antibody (lanes 4 and 5) or control antibody (lanes 6 and 7). RNA was analyzed immediately (lane 1) or 40 min (lanes 2–7) after injection. (B) Quantitation of RNA export from the four independent experiments as in A. (C) The same as A except that human SRP RNA was used and pre-miR-31 was omitted, and the incubation was 0 or 1.5 h. (D) Quantitation of RNA export from the three independent experiments as in C. Image published in: Takeiwa T et al. (2015) © 2015 The Authors. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives license Larger Image Printer Friendly View

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