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Fig. 2. Representative phenotypes of F0 embryos injected with TALENs. Lateral (A and B) and dorsal (A′, the same embryo shown in A) views of two embryos injected with a pair of TALENs targeting pax6 exon 9 (200 pg mRNA each). Asterisks in (A) and (A′) indicate the same abnormal eye. The most frequently seen eye phenotype is shown in (B) (arrow). (C) Lateral view of an embryo injected with a pair of TALENs targeting tyr (200 pg mRNA each). The eye is morphologically normal but less pigmented (arrow). (D) Lateral view of an embryo injected with two pair of TALENs targeting pax6 exons 7 and 9 (200 pg mRNA each). The abnormality here (arrow) is similar to what is shown in (B). (E) Mating of mature frogs raised from embryos injected with two pairs of pax6 TALENs. Eyes are missing in one side of each animal (arrows).

Image published in: Nakayama T et al. (2015)

Copyright © 2015. Image reproduced on Xenbase with permission of the Publisher, Elsevier B. V.

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