XB-IMG-140027
Xenbase Image ID: 140027
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Figure 1. nodal1 Rescues Cilia Defects
Induced by Notch1 Knockdown
(A) Cilia length at the GRP. N1MO (50 ng), NICD
RNA (100 pg), nodal1 RNA (5 pg), and/or conMO
(50 ng) were injected into the dorsal marginal
zone of four-cell embryos, and embryos were
fixed at stage 17. Cilia were stained using an antiacetylated
a-tubulin antibody. Membrane RFP
(memRFP) RNA was co-injected as a lineage
tracer and to indicate the cell membrane. Scale
bar represents 15 mm. a, anterior; p, posterior.
(B) Average length of cilia from (A). Fifty cilia
from six GRPs were analyzed for each sample.
Two GRPs were randomly chosen from each of
three independent experiments.
(C and D) Leftward flow. GRPs were explanted
at stage 17, and flow was imaged subsequent to
the addition of fluorescent beads. Velocity (C) is
shown in mm/s, and directionality (D) is shown as
the dimensionless number rho. The maximum
value r = 1 indicates that all trajectories point in the
same direction, whereas a r value of 0 indicates
uniform distribution in all directions.
Statistical analyses were carried out in Excel using
SEM and Student’s t test to calculate p values
(*p % 0.05, **p % 0.005, ***p % 0.0005, ****p %
0.00005; n.s., not significant). Image published in: Tözser J et al. (2015) Copyright © 2015. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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