XB-IMG-140082
Xenbase Image ID: 140082
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Fig 8. Reduction of cell densities by co-knockdown of nemp1 and ran. (A) A. Effects of nemp1MOs on cell densities. Dorsoanterior views (a, b, c, d, e, f)
of embryos are shown. Yellow boxes in a, b, d, and e correspond to enlarged areas a’, b’, d’, and e’, respectively. Upper panels, stdMO (40 ng/embryo); lower
panels, nemp1MOs and stdMO (20 ng each/embryo) (the same experiment as in panel B). Embryos were injected with MOs and FITC-dextran as a tracer,
fixed at stages 12.5–13, and immunostained with anti-phospho histone H3 antibody (red). DAPI was used for nuclear staining. White arrowheads, positions of blastopores; magenta scale bars, 500 μm; white scale bars, 100 μm. (B)B. Synergistic effects of nemp1MOs and ranMOs on cell densities. Combinations
of MOs and amounts (ng/embryo) are as indicated. Experiments were repeated three times and similar results were obtained, one of which is presented here.
DAPI-stained nuclei were counted in FITC-positive areas. C,D. Rescue of reduced cell density in morphants by mRNA injection. Combinations of MOs and
mRNAs as well as amounts of MO (ng/embryo) and mRNA (pg/embryo) are as indicated. Injected embryos were fixed and immunostained using anti–HA
antibody. DAPI-stained nuclei were counted in EGFP-HA positive areas. (E) E. Reduction of cell densities by overexpression of Nemp1. Injected mRNA and
amount (pg/embryo) are as indicated. DAPI-stained nuclei were counted in EGFP-HA positive areas. *, P<0.05; ***, P<0.005; error bars,
standard deviation. Image published in: Shibano T et al. (2015) Image reproduced on Xenbase with permission of the publisher and the copyright holder. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license
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