Xenbase Image ID: 140083

Fig 9. Evolutionary conservation of Ran binding of region B in Arabidopsis. A. Co-IP of At_Nemp and At_Ran. Because region B of At_Nemp proteins are not well defined by comparison to vertebrate Nemp1, which is attributed to low amino acid conservation, the entire C-terminal regions downstream of the last TM (named Ct, see S7 Fig C) were used for co-IP experiments. mRNA for GFP-tagged At_Ct constructs were injected with mRNA for Myc-tagged At_Ran2 into Xenopus embryos. B. Co-IP of At_Nemp with Mm_Ran or Mm_Nemp1 with At_Ran2. Xenopus embryos were coinjected with combinations of mRNAs as indicated. Experimental conditions were the same as in Fig 3. Black arrowhead, expected product bands; white arrowheads, cross-reacted bands. After immunoprecipitation against HA, western blotting was performed with anti-Myc or HA antibody as indicated. Image published in: Shibano T et al. (2015) Image reproduced on Xenbase with permission of the publisher and the copyright holder. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

Return to previous page