Xenbase Image ID: 140086

S3 Fig. Co-IP of Nemp1 with Ran using Xenopus embryos. This is the original data for Figs 5A and 6D. mRNA for HA-tagged Mm_Nemp1 or its mutants (5SA, 5SE) was coinjected into Xenopus embryos with mRNA for a Myc-tagged construct of Mm_Ran (WT) or its mutants (T24N, Q69L, T42A, δC). Injected embryos were collected at the mid blastula stage (stages 8–8.5) and lysed with lysis buffer B for Co-IP. Black arrowheads, modified forms of Nemp1- HA. Note that WT Nemp1 has two major modified bands (lane 2) and co-expression with Ran(T24N; a GDP form) enhanced these modifications (lane 3). Also note that the upper modified band disappeared in 5SA and 5SE constructs (lanes 7, 8), suggesting that all or some of these five serine residues are involved in modification (phosphorylation) by functioning as either phosphorylation sites or recognition sites or both, and that there are other phosphorylation sites besides there five serine residues. (TIF) Image published in: Shibano T et al. (2015) Image reproduced on Xenbase with permission of the publisher and the copyright holder. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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