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S6 Fig. Gain- and loss-of-function experiments for the ratio of mitotic cells. Combinations
of injected MOs and mRNAs as well as amounts of MO (ng/embryo) and mRNA (pg/embryo)
are as indicated. Experiment conditions are the same as in Fig 8. (A) Reduction of the ratio of
mitotic cells by co-knockdown of Nemp1 and Ran. Similar tendencies were obtained from the
three experiments and statistically significant differences was observed in one of them. Nuclei
stained with DAPI or immunostained for phospho histone H3 were counted in FITC-positive areas. (B) Reduction of the ratio of mitotic cells by overexpression of Nemp1. DAPI-stained
nuclei were counted in EGFP-HA positive areas. , P<0.05; , P <0.005; error bars,
standard deviation.
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