XB-IMG-140107
Xenbase Image ID: 140107
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Fig. 4. Caged serotonin molecules implicate 5-HT in LR patterning of
a secondary organizer. (A) In singleton embryos, injection of the caged
molecule (BHQ-O-5HT) at the 1-cell stage, paired with uncaging at the
32-cell stage, induces significant levels of heterotaxia. Photoactivation of
5-HT at the 8-cell stage did not significantly increase rates of LR patterning
defects. (B) BHQ-O-5HT was injected at the 1-cell stage and conjoined
twins were induced at the 8- or 16-cell stage. When 5-HT was uncaged at
the 32-cell stage, prior to the induction of conjoined twins, LR patterning
in the induced twin was randomized. When 5-HT was uncaged at stage
8, as XSiamois begins to induce the development of a conjoined twin, LR
patterning defects were still observed. (C) BHQ-O-5HT and XSiamois were
co-injected in the ventral vegetal left blastomere at the 8- or 16-cell stage,
and 5-HT was uncaged at either 32-cell, stage 8, or stage 10. Regardless
of when the 5-HT was released, heterotaxia in the induced twin was
observed. In all panels, the numbers on the graphs indicate the sample
size, *p<0.05 compared to embryos injected with BHQ-O-5HT but not
uncaged, Chi Square test. Red bars indicate inverted hearts, blue indicates
inverted stomach and/or gall bladder, and purple indicates inverted heart
plus stomach and/or gall bladder. Image published in: Vandenberg LN et al. (2014) Copyright © 2014. Reproduced with permission of the Publisher, University of the Basque Country Press. Larger Image Printer Friendly View |