Xenbase Image ID: 140108

Fig. 5. In situ hybridization for collagen9a2 confirms the microarray result of left sided expression prior to nodal flow. (A) Embryos processed by in situ hybridization for the marker col9a2 show expression only on the left side or (B) on both sides but with a strong left bias. Embryos were examined prior to the development of ciliary flow. (C) Control embryos probed for the marker Xslug, which was not biased in the microarray results, showed bilateral symmetric expression in all animals examined. Red arrows indicate areas of expression; white arrows indicate absence of expression. The dotted line demarcates the embryo’s midline. All panels show a dorsal view, with the anterior pointing upward. Image published in: Vandenberg LN et al. (2014) Copyright © 2014. Reproduced with permission of the Publisher, University of the Basque Country Press. Gene Synonyms Species Stage(s) Tissue col9a2.L X. laevis Sometime during NF stage 12 to NF stage 13 left non-neural ectoderm snai2.L slug, snai2-a, snai2-b, Snail2, xSlu, xslug, XSnail2 X. laevis Sometime during NF stage 12 to NF stage 13 cranial neural crest Image source: Published Larger Image Printer Friendly View

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