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XB-IMG-140110

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Supplementary Fig. 1. b-gal localization in early conjoined twins verifies RNA translation. (A) Embryos were injected with b-gal mRNA at 1-cell stage and XSiamois at 8-16 cell stage. Embryos fixed at the indicated stages (st. 10 – 12.5) express b-gal, indicating that RNA translation occurs. (B) One blastomere was injected with b-gal mRNA at the 2-, 4- or 8-cell stage, and embryos were injected with XSiamois at 8-16 cell stage. Different patterns of b-gal expression were observed in developing twins at neurula stages depending on the original location of the b-gal mRNA injection.

Image published in: Vandenberg LN et al. (2014)

Copyright © 2014. Reproduced with permission of the Publisher, University of the Basque Country Press.

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