Xenbase Image ID: 140128

Figure 1. Analysis of Xenopus peripherin-2-GFP fusion protein. (A) Topological model for Xenopus peripherin-2 showing the location of the GFP fusion protein, the epitope for the Xper5A11 mAb, mutations analyzed in this study (white circles), and amino acids that differ from the published sequence (black circles). (B) Western blots of Xenopus peripherin-2-GFP expressed in COS-1 cells. COS-1 membranes were preincubated with or without DTT, solubilized in Triton X-100, treated with or without glutaraldehyde (Glut), and fractionated on SDS gels under reducing (+BME, β-mercaptoethanol) or nonreducing (-BME) conditions. Lanes a–d: WT Xenopus peripherin-2-GFP labeled with an anti-GFP antibody. Lane e: C150S Xenopus peripherin-2-GFP labeled with anti-GFP antibody. Lane f: Coexpression and coimmunoprecipitation of Xenopus peripherin-2-GFP (XPerGFP) and bovine peripherin-2 (BPer) with bovine specific Per2B6-Sepharose. Western blots were labeled with an anti-GFP antibody to detect XperGFP and the Per2B6 antibody to detect BPer. Image published in: Loewen CJ et al. (2003) Copyright © 2003. Image reproduced with permission of the Publisher, American Society for Cell Biology (ASCB). This is an Open Access article. Larger Image Printer Friendly View

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