XB-IMG-140129
Xenbase Image ID: 140129
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Figure 2. Endogenous peripherin-2 and WT peripherin-2-GFP localize to the ROS disk rims and incisures. (A and B) X. laevis retina was labeled with TR-WGA (red) and antiperipherin-2 Xper5A11 antibody (green) and stained with Hoescht 33342 (blue). (A) TR-WGA labeling of glycoproteins in rod (ros) and cone (cos) outer segments; inner segment (is) and nuclei (n) are not labeled; (B) peripherin-2 labeling along the disk rims and incisures (arrowheads) and one edge of the cos (arrow). Bar, 5 μm. (C–E) Cryosections of 4-week-old tadpole eyes expressing WT Xenopus peripherin-2-GFP (green) and stained with TR-WGA (red) and Hoescht 33342 (blue). (C) Retina expressing Xenopus peripherin-2-GFP in ROS. Fusion protein is also present in phagosomes in retinal pigment epithelial cells (arrow). l, lens; r, retina; rpe, retinal pigment epithelium; Bars, 100 μm. (D and E) Micrographs of retinas expressing Xenopus peripherin-2-GFP at higher magnification showing peripherin-2-GFP localized to the ROS disk rims and incisures (arrowheads). Bars, 10 μm (D) and 5 μm (E); (Inset) Cross section of a ROS. Bar, 1 μm. All detectable WT peripherin-2-GFP localized to the ROS. F-H. Electron micrographs of retina labeled for Xenopus peripherin-2-GFP with an anti-GFP antibody and immunogold markers. (F) Longitudinal section (Bar, 0.2 μm) and (G) transverse section (Bar, 0.5 μm) of a rod expressing moderate levels of fusion protein. Peripherin-2-GFP is present on the disk rims (arrows) and incisures (arrowheads). (H) Composite longitudinal section of a ROS expressing high levels of the fusion protein. Some missorting of the fusion protein to the disk lamellae and constriction of the rod outer segment is evident. Bar, 0.5 μm. Image published in: Loewen CJ et al. (2003) Copyright © 2003. Image reproduced with permission of the Publisher, American Society for Cell Biology (ASCB). This is an Open Access article.
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