Xenbase Image ID: 140133

Figure 6. Expression and biochemical properties of Xenopus peripherin-2-GFP in cultured COS cells. (A) Confocal micrographs of COS cells expressing WT, C214S, or P216L Xenopus peripherin-2-GFP (XPer-GFP) and labeled with an anticalnexin antibody as an ER marker. WT peripherin-2 and a portion of the mutants are present in intracellular vesicles that do not label for calnexin. A significant fraction of the C214S mutant as well as L216P, however, is retained in the ER. (B) Membranes from cells transfected with wild-type (WT), C214S or P216L peripherin-2-GFP were solubilized and incubated in the presence or absence of PNGase. Peripherin-2 was resolved by SDS gel electrophoresis and detected on Western blots labeled with an anti-GFP antibody. A small shift in glycosylated (gly) WT and C214S peripherin-2 and hyperglycosylated (hgly) P216L peripherin-2 to its deglycosylated (dgly) form is observed after treatment with PNGase. Image published in: Loewen CJ et al. (2003) Copyright © 2003. Image reproduced with permission of the Publisher, American Society for Cell Biology (ASCB). This is an Open Access article. Larger Image Printer Friendly View

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