Xenbase Image ID: 144157

Fig. 3. ATP4-dependent Wnt signaling is required for foxj1 expression and ciliation in the Xenopus embryonic skin. (A–E) Immunofluorescent analysis of skin ciliation at stage 30. Embryos were stained for tubulin (acetylated-α-tubulin, red) and actin (phalloidin-Alexa488, green) and analyzed by confocal microscopy. (B) In atp4a morphants fully ciliated, partially ciliated and non-ciliated MCCs were found. Smaller intercalating cells with ISC morphology, which are also negative for acetylated-α-tubulin staining are marked with asterisks in A and B. Note that atp4a (C), β-catenin (β-cat.; D) and foxj1 (E) DNAs partially rescued ciliation in atp4a morphants. (A‵–E‵) Lateral projections of confocal z-scans are shown in (A)–(E). (A‶–E‶) Higher magnification of representative MCCs. (F) Quantification of results (n, number of embryos; (n), number of cells). Image published in: Walentek P et al. (2015) Copyright © 2015. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue tuba4b.L alpha tubulin, alpha-tubulin, tuba4, tuba4a X. laevis Throughout NF stage 29 and 30 epidermis ciliated epidermal cell Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + atp4a MO + NF29/30 (immunohistochemistry) Fig. 3 A B F Anatomical Phenotype abnormal cilium assembly Disease pneumonia Larger Image Printer Friendly View

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