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S2 Expression of three major transcriptional regulators in MCCs was analyzed: multicilin (mci) ( Stubbs et al., 2012)⁠, myb (Tan et al., 2013)⁠ and foxj1 ( Fig. S2C). mci was only weakly expressed at stage 10, peaked at stage 19 and was barely detectable at later stages (25 and 32) of mucociliary development. Image published in: Walentek P et al. (2015) Copyright © 2015. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue foxj1.L foxj1-a, foxj1-b, hfh-4, hfh4, xfoxj1 X. laevis Throughout NF stage 15 epidermis non-neural ectoderm foxa1.L fkh2, foxa1-a, foxa1-b, foxa1b, HNF-3 alpha, HNF3 alpha, HNF3-alpha, hnf3a, HNF3alpha, tcf3a, tFoxA1, xfkh2 X. laevis Throughout NF stage 18 ciliated epidermal cell foxi1.L ectodermally-expressed mesendoderm antagonist, ema, FoxI1e, foxi1e, foxi3, Xema, xfoxi1 X. laevis Throughout NF stage 18 ciliated epidermal cell atp6v1e1.L atp6e, atp6v1e, vma4 X. laevis Throughout NF stage 32 ionocyte Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + atp4a MO + NF15-18 (in situ hybridization) Fig. S 2 A Disease pneumonia Expression Phenotype decreased amount foxj1.L expression in epidermis Xla Wt + atp4a MO + NF15-18 (in situ hybridization) Fig. S 2 B Disease pneumonia Expression Phenotype decreased amount foxa1.L expression in epidermis Larger Image Printer Friendly View

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