Xenbase Image ID: 144188

Figure 4. Cilia targeting of P/rds in cilia is dependent on COPII, but not on the GRASP55-mediated mechanism. A, Cells stably expressing bP/rds that are also transiently expressing either wild-type or the H79G mutant of Sar1 (red) were colabeled with anti-GRASP55 (green). Expression of Sar1(H79G), but not wild-type Sar1, caused the disruption of cis-Golgi structure. B, P/rds (green) was colabeled with wild-type or the H79G mutant of Sar1 (red). The primary cilia is also labeled by anti-acetylated tubulin (red, the same color as Sar1). Cilia targeting of P/rds was inhibited by Sar1(H79G). C, P/rds (green) was colabeled for Sar1 (blue) and calreticulin (red, an ER marker). P/rds localized in ER in the cell expressing Sar1(H79G). D, E, Cells stably expressing bP/rds were transfected with GRASP55 siRNA and then analyzed for GRASP55 protein level (D) and the localization of P/rds (E; green) in primary cilia (acetylated tubulin, red). The siRNA effectively reduced the protein expression of GRASP55 by more than 10-fold, but did not affect the cilia targeting of P/rds. The images are confocal images of a single x–y plane. Scale bars, 10 μm. Image published in: Tian G et al. (2014) Copyright © 2014. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

Return to previous page