Xenbase Image ID: 144189

Figure 5. The C-terminal tail region of P/rds is essential for the cilia targeting. A, hTERT-RPE1 cells transiently expressing full-length or CT-truncated bP/rds were analyzed for the percentage of the primary cilia positive to P/rds. Strong (black bar), bP/rds is enriched in cilia; weak (red bar), bP/rds is in cilia but not enriched; NA (green bar), P/rds is not observed in cilia. ***p < 0.001 compared with bP/rds by t test. Error bars represent SD. bP/rds (1060 cilia from n = 6 preparations), bP/rds1-342 (635 cilia from n = 3 preparations), bP/rds1-336 (416 cilia from n = 3 preparations), bP/rds1-330 (245 cilia from n = 3 preparations), bP/rdsΔ289-312 (501 cilia from n = 3 preparations). A gradual reduction in the cilia-targeting efficacy was observed by CT truncations. B, The alignment of the P/rds CT tail region indicates that this region is highly conserved among vertebrate species: identical (*) or homologous (:) residues are shown. C, bP/rds1-342 (green) was concentrated in cilia like full-length protein. bP/rds1-336 (green) and bP/rdsΔ289-312 (green) localized in cilia but were not enriched and bP/rds1-330 (green) did not localize in cilia. Acetylated tubulin (red) was used as a cilia marker. The images are confocal images of a single x–y plane. Scale bar, 10 μm. Image published in: Tian G et al. (2014) Copyright © 2014. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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