Xenbase Image ID: 144190

Figure 6. C-terminal tail region of P/rds is essential for Golgi retention. A–C, hTERT-RPE1 cells transiently expressing full-length or CT-truncated bP/rds (green) were colabeled for GM130 (A; cis-Golgi), for GMII (B; medial and trans-Golgi), or for P230 (C; TGN). The images are confocal images of a single x–y plane. D, Maximum projection images of bP/rds and CT truncations. bP/rds is concentrated in cilia when the expression level is low (arrow) and localized to Golgi and other parts of cells except cilia when the expression level is high (arrowhead). CT truncation, down to the position 312, led to the accumulation of P/rds in the Golgi apparatus. Further truncation to position 288 led to exit of P/rds from the Golgi. Scale bars, 10 μm. E, CT-truncated bP/rds transiently expressed in hTERT-RPE1cells were treated with PNGase F (P) or Endo H (En). Truncation of the CT tail led to acquisition of Endo H resistance. Therefore, P/rds is capable of serving as a substrate for the GMII. Image published in: Tian G et al. (2014) Copyright © 2014. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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