Xenbase Image ID: 144191

Figure 7. Unconventional cilia targeting of P/rds in IMCD3 cells. A, hTERT-RPE1and IMCD3 cells stably expressing bP/rds or bP/rds1-288 were separated into cytoplasm (Cyto) and plasma membrane (PM) fractions by the biotinylation method and analyzed by Western blots. bP/rds1-288 is barely observed in the plasma membrane. Na+/K+ ATPase (bottom) was used to demonstrate that the plasma membrane proteins were pulled down effectively. B, PNGase F (P) and Endo H (En) treatments of IMCD3 cells stably expressing bP/rds or its truncation mutants. C, Maximum projection images of full-length and CT-truncated bP/rds expressed in IMCD3 (green). The arrow indicates the cilium. D, Top, IMCD3 and hTERT-RPE1 cells stably expressing bP/rds1-312 (HA, green) were labeled for a cis-Golgi marker GM130 (red). Bottom, Intensity of bP/rds1-312 and GM130 (arbitrary intensity units, AI) along the white bars shown in the top. Significant overlap between bP/rds1-312 and cis-Golgi was observed in IMCD3 cells, whereas the overlap is less significant in hTERT-RPE1 cells. Scale bars, 10 μm. Image published in: Tian G et al. (2014) Copyright © 2014. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

Return to previous page