Xenbase Image ID: 144195

Figure 11. Model for unconventional cilia targeting of P/rds. A, The summarized localizations of P/rds and the CT truncations in photoreceptor cells and mammalian cells. ++, +, +/−, and − indicate that the transgenes' signals were strong, weak, very weak, and undetectable, respectively. Note: When overexpressed in mammalian cells, P/rds can also be detected in Golgi and other intracellular compartments. B, In both mammalian cells (left) and X. laevis rods (right), P/rds bypasses the Golgi apparatus, either the later portions or altogether, to reach the cilia, thus taking an unconventional secretory pathway (green arrow, P/rds). The Golgi retention signal prevents conventional secretion of P/rds1-312 or P/rds1-316 (red arrow, P/rds1-312 or P/rds1-316), but does not block unconventional cilia targeting in rods (red dash-dotted arrow), likely due to trafficking driven by oligomerization with endogenous P/rds. After release from Golgi (left, blue dotted arrow, P/rds1-288), P/rds1-288 is incapable of taking the secretory pathway in mammalian cells (left, blue arrow). Routes labeled 1 and 2 are the estimated routes explaining the cell-line-dependent variability observed between hTERT-RPE1 and IMCD3 cells. Neither 1 or 2 is a complete secretory pathway and cannot deliver P/rds1-288 to the cell surface. P/rds1-288 can reach the cilium through unconventional secretion in rods (right, blue dotted and dash-dotted arrows). Image published in: Tian G et al. (2014) Copyright © 2014. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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