Xenbase Image ID: 144199

Figure 3 A role for Prdm12 in sensory neuron development. (d) Knockdown of Prdm12 by a specific morpholino (MO) in Xenopus embryos caused irregular staining for markers of cranial sensory placode development (Ath3, Ebf3 and Islet1). Embryos injected with control MO or Prdm12 MO were analyzed at the late tailbud stage (stage 28) by whole-mount in situ hybridization; yellow arrowheads, profundal placode; green arrowheads, trigeminal placode. Scale bars, 200 μm. Normal gene expression domains of cranial placodes in Xenopus laevis are shown in the schematic drawing at the top of the panel (lateral view, late tailbud stage; modified from ref. 25). The results were categorized and quantified (n ≥ 46 live embryos per condition). Statistical differences between expression in control MO–treated and Prdm12 MO–treated embryos are indicated. ***P < 0.001 (two-sided Mann-Whitney U-test). Image published in: Auer-Grumbach M et al. (2015) Copyright © 2015. Image reproduced with permission of the Publisher, Macmillan Publishers Ltd. Experiment + Assay Source Phenotypes and Disease Xla Wt + prdm12 MO + NF28-28 (in situ hybridization) Fig.3.d Expression Phenotype decreased amount ebf3.L expression in neurogenic placode decreased amount isl1.S expression in neurogenic placode decreased amount neurod4.L expression in neurogenic placode Larger Image Printer Friendly View

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